Teaching Exercises

These are teaching exercises that can be done using reagents from FR3 and BEI.

se are teaching exercises that can be done using reagents from FR3 and BEI.
Differential filarial gene expression by real-time PCR
Requires use of Brugia malayi adult male and female RNA, available through BEI Resources. cDNA synthesis can be set up within 30 minutes, followed by a 45 minute incubation. Quantitative RT-PCR requires a 30 minute setup and a 2.5 hour cycle time.
Immunohistochemistry for Wolbachia surface protein
We perform this exercise at the annual FR3 minicourse. It has been optimized to take only 2-3 hours if slides are deparaffinized beforehand. Note: this requires the anti-WSP monoclonal antibody, BEI Resources BEI catalog # NR-31029.
HATCH OF AEDES AEGYPTI EGGS