Parasite Protocols

Research Protocols

SOP NUMBER

TITLE.

8.2 Mosquito Rearing Techniques
8.3 Infecting Aedes aegypti with Brugia pahangi, B. malayi, and Dirofilaria immitis
8.4 Collecting Infective Larvae of Brugia pahangi, B. malayiDirofilaria immitis, and Dirofilaria repens From Infected Mosquitoes
8.5 Experimental Infection of Dogs, Cats, Ferrets, and Rodents with Dirofilaria immitis, BrugiamalayiB. pahangi, or Dipetalonema reconditum
8.31 Standard Diet for Rearing Mosquitoes
8.56 Necropsy for Dirofilaria immitis in Dogs, Cats, and Ferrets
8.94 The Collection of Brugia sp. from Jirds, and the Surgical Transplantation of Adult Brugia sp. into Jirds
 UWO1 Purification of Brugia mf by density gradient centrifugation
 UWO2 Purification of Brugia mf by filtration
UWO3 Counting microfilariae
SWDNA1 Smit  h DNA Isolation from filarial nematodes
 SWRNA1 Smith RNA Isolation from filarial nematodes
   JFRNA2 RNA isolation protocol for samples with high level blood contamination
 SWPCR1 Filarial PCR and RT-PCR (Tph-1 gene example)
 SWEST1  Filarial Genome Project EST generation protocols
 SWMD1 Molecular Diagnostics Protocol
 AVIT1 Collection of A. viteae adults from hamsters
AVIT2 Culture of Infective Larvae or Adults of Acanthocheilonema viteae
 AVIT3 Infecting Ornithodoros tartakowskyi with Acanthocheilonema viteae
 AVIT4 Retro-orbital eye bleeds of gerbils and hamsters
 AVIT5 Microfilariae enumeration of A. viteae from rodent peripheral blood samples
 AVIT 6 Collecting Infective Larvae of Acanthocheilonema viteae From Infected Ticks
 AVIT7 Surgical Transplantation of Adult A. viteae into Jirds
 A. vit 8 Experimental Infection of Gerbils and Hamsters with Acanthocheilonema viteae L3
 A. vit 9

A. vit 10

 Maintenance of Ornithodoros tartakowskyicolony

Tick Rearing Techniques Using Commercially-Obtained Blood (Artificial Membrane Feeding)

WSP Ab Wolbachia surface protein antibody (IHC) protocol