FR3 Molecular Protocols

Filarial RNA Isolation – click here for Williams lab protocol for the ‘BB’ method of total RNA isolation.

Alternate RNA isolation protocol – developed in the Christensen lab for samples with high levels of blood contamination (i.e. microfilariae).

Filarial PCR and RT-PCR – click here for FR3 workshop exercise for amplifying the Tph-1 gene from DNA by PCR, and from total RNA by RT-PCR.

EST generation – click here for Filarial Genome Project protocols.

Molecular Diagnostics Protocols – click here for references to PCR and RT-PCR – based diagnostic tests for detection and differentiation of filarial parasites in blood and mosquitoes.